体外铁死亡和铜死亡研究中铁离子和铜离子来源

体外铁死亡和铜死亡研究中铁离子和铜离子来源

Sources of iron and copper in the study of ferroptosis and cuproptosis in vitro

问：有客户在文章审稿时被一位审稿人问道：在细胞中研究铁死亡时铁离子的来源是什么？

答：常规的细胞培养基中都含有铁离子，因此在细胞中研究铁死亡时铁离子来源是细胞培养基。类似的，在细胞中研究铜死亡时铜离子来源是血清，因为血是铜离子载体，这在首次报道铜死亡的文章中已经验证过¹。

Question: A client was asked by a reviewer during the article review process: What is the source of iron when studying ferroptosis in cells?

Answer: Conventional cell culture media contain iron, so the source of iron is the cell culture medium when studying ferroptosis in cells. Similarly, the source of copper is serum when studying cuproptosis in cells as blood is the carrier of copper, which has been validated in the first article reporting cuproptosis¹.

参考文献（Reference）

1    Tsvetkov, P. et al. Copper induces cell death by targeting lipoylated TCA cycle proteins. Science 375, 1254-1261 (2022). https://doi.org:10.1126/science.abf0529