如何预测蛋白质琥珀酰化位点

如何预测蛋白质琥珀酰化位点

How to predict succinylation sites on proteins

利用PhosphoSitePlus预测蛋白质琥珀酰化位点，步骤如下：

The steps for predicting succinylation sites on proteins by PhosphoSitePlus are as follows:

1 从https://www.phosphosite.org/homeAction网址进入PhosphoSitePlus

1 Enter PhosphoSitePlus from https://www.phosphosite.org/homeAction

2输入靶蛋白质名称（SUCLG2），搜索

2 Input target protein name (SUCLG2), search

3 选择物种（人）

3 Select species (Human)

4 点击“Site Table”

4 Click “Site Table”

5 查看结果

5 View results

6 点击位点查看详情

6 Click the site for details

PhosphoSitePlus也可用于预测特定多肽的琥珀酰化位点，步骤如下：

The steps for predicting succinylation sites on specific peptides by PhosphoSitePlus are as follows:

1 从https://www.phosphosite.org/homeAction网址进入PhosphoSitePlus

1 Enter PhosphoSitePlus from https://www.phosphosite.org/homeAction

2 点击“Site Search”

2 Click “Site Search”

3 输入多肽序列，搜索

3 Input the peptide sequence, search

4 查看结果

4 View results

5 点击位点查看详情

5 Click the site for details

另外，还可以利用CPLM（Compendium of Protein Lysine Modifications）预测蛋白质琥珀酰化位点，步骤如下：

Additionally, CPLM also could be used to predict succinylation sites on proteins, and steps are as follows:

1从https://cplm.biocuckoo.cn/index.php网址进入CPLM

1 Enter CPLM from https://cplm.biocuckoo.cn/index.php

2输入基因名称（SUCLG2），提交

2 Input gene name (SUCLG2), submit

3 选择物种（人），点击“CPLM ID”查看结果

3 Select species (Homo sapiens), click “CPLM ID” to view results

4 点击“View”查看详情

4 Click “View” for details

5 或点击“UniProt Accession”查看结果

5 Or click “UniProt Accession” to view results

6 点击“PTM/Processing”查看结果

6 Click “PTM/Processing” to view results

7查看结果

7 View results