如何预测RNA与蛋白质的互作

如何预测RNA与蛋白质的互作

How to predict the interaction of RNA and protein

      RNA需要与蛋白质协同合作发挥作用，且RNA与蛋白质两者间可相互调控。因此，预测RNA与蛋白质的互作对于机制研究十分重要。本文将讲解如何预测RNA与蛋白质的互作。

RNAs exert functions by collaborating with proteins, and RNAs and proteins could regulate each other. Therefore, prediction of RNA-protein interaction is critical for mechanical study. This article shows how to predict the interaction of RNA and protein.

对于已知的RNA与蛋白质的互作，可以利用ENCORI/Starbase挖掘，步骤如下：

For known RNA-protein interaction, ENCORI/Starbase could be used to dig, and steps are as follows:

1 从https://rnasysu.com/encori/index.php网址进入ENCORI/Starbase

1 Enter ENCORI/Starbase from https://rnasysu.com/encori/index.php

2 点击RBP-Target，选择RBP-mRNA/caRNA/lncRNA/sncRNA/circRNA（本文选择RBP-mRNA）

2 Click RBP-Target，select RBP-mRNA/caRNA/lncRNA/sncRNA/circRNA (RBP-mRNA is selected in this article)

3 选择分支（哺乳类）

3 Select a clade (mammal)

4 输入mRNA名称，快速搜索

4 Enter the mRNA name, quick search

5查看结果

5 View results

6 或者输入蛋白质名称，快速搜索

6 Or enter the protein name, quick search

7 查看结果

7 View results

对于未知的RNA与蛋白质的互作，可以利用RNA-Protein Interaction Prediction (RPISeq)进行预测，步骤如下：

For unknown RNA-protein interaction, RNA-Protein Interaction Prediction (RPISeq) could be used to predict, and steps are as follows:

1 从http://pridb.gdcb.iastate.edu/RPISeq/网址进入RNA-Protein Interaction Prediction (RPISeq)

1 Enter RNA-Protein Interaction Prediction (RPISeq) from http://pridb.gdcb.iastate.edu/RPISeq/

2 分别输入蛋白质和RNA序列，提交

2 Input protein sequence and RNA sequence, respectively, submit

3 查看结果，相互作用概率大于0.5被认为预测RNA与蛋白质互作可能性大

3 View results，interaction probabilities > 0.5 indicates that the corresponding RNA and protein are likely to interact