如何预测RNA甲基化位点

如何预测RNA甲基化位点

How to predict methylation sites on RNA

        RNA甲基化是指将甲基基团共价添加到RNA核苷酸上的修饰方式，对RNA稳定性、定位、运输、剪切和翻译等发挥重要的作用。在研究RNA甲基化之前，如果能先预测RNA甲基化位点，后面的研究将事半功倍。本文将讲解如何预测RNA甲基化位点。

RNA methylation refers to the covalent addition of methyl groups to nucleotides of RNA, playing a crucial role in RNA stability, location, transport, splice and translation. If RNA methylation sites can be predicted, subsequent research would be twice as efficient. This article shows how to predict methylation sites on RNA.

RNA甲基化包含多种类型，包括m6A，m1A，m5C，hm5C，m7G，2’-O-me和psi等。具体的RNA甲基化位点可通过RMVar 2.0进行预测，步骤如下：

There are various types of RNA methylation, including m6A, m1A, m5C, hm5C, m7G, 2’-O-me and psi. RMVar 2.0 could be used to predict specific RNA methylation sites, and steps are as follows:

1 从https://rmvar.renlab.cn/#/home?pid=6网址进入RMVar 2.0

1 Enter RMVar 2.0 from https://rmvar.renlab.cn/#/home?pid=6

2 选择“Gene name”，输入基因名称（NAT10），搜索

2 Select “Gene name”, input gene name (NAT10), search

3 选择修饰类型（m6A），搜索

3 Choose modification type (m6A)，search

4 查看结果

4 View results

5 点击“RMVar ID”查看详情

5 View details by clicking “RMVar ID”

        RNA甲基化是一个动态可逆的过程，主要由三类蛋白质协同调控：甲基转移酶（Writer）负责添加甲基基团，去甲基化酶（Eraser）负责去除甲基基团，甲基识别蛋白（Reader）通过识别并结合甲基基团调控RNA的功能。因此，如果能挖掘潜在的Writer、Eraser和Reader，后面的研究将事半功倍。RNA甲基化的Writer、Eraser和Reader可通过RM2Target^v2.0进行挖掘，步骤如下：

RNA methylation is a dynamic and reversible process primarily regulated by three classes of proteins: methyltransferases (Writers) responsible for adding methyl groups, demethylases (Erasers) for removing methyl groups, and methyl-binding proteins (Readers) that modulate RNA function by recognizing and binding methyl groups. Therefore, dig potential Writers, Erasers, and Readers can significantly enhance subsequent research efforts. Writers, Erasers, and Readers for RNA methylation could be identified by RM2Target^v2.0, and steps are as follows:

1 从http://rm2target.canceromics.org/#/home网址进入RM2Target^v2.0

1 Enter RM2Target^v2.0 from http://rm2target.canceromics.org/#/home

2 选择“Target gene”，输入基因名称（NAT10），搜索

2 Select “Target gene”, input gene name (NAT10), search

3 查看结果

3 View results

4 点击“RM2Target ID”查看详情

4 View details by clicking “RM2Target ID”

5 如果想确定具体的Writer、Eraser和Reader是否调控靶RNA，点击“WERs”-选择物种（Homo sapiens）-选择修饰类型（m6A）-选择蛋白质类型（Writer）-选择具体蛋白质（METTL14），搜索

5 If you want to identify whether specific Writer, Eraser or Reader regulates target RNA, click “WERs”-choose species (Homo sapiens)-choose modification type (m6A)-choose protein type (Writer)-choose specific protein (METTL14), search

6 查看结果

6 View results

7 点击“RM2Target ID”查看详情

7 View details by clicking “RM2Target ID”