如何预测蛋白质乳酸化位点

如何预测蛋白质乳酸化位点

How to predict lactylation sites on proteins

       乳酸化/乳酰化是一种新近发现的与代谢相关的蛋白质修饰，指将来源于乳酸的乳酰基团通过酶促转移到蛋白质赖氨酸残基上。组蛋白乳酸化/乳酰化在调控基因转录活性中发挥重要的作用。非组蛋白乳酸化/乳酰化则参与调控蛋白质活性和互作。乳酸化/乳酰化writer蛋白（p300）促进蛋白质乳酸化/乳酰化，而乳酸化/乳酰化eraser蛋白（HDAC1-3/ SIRT1-3）则抑制蛋白质乳酸化/乳酰化。在研究蛋白质乳酸化之前，如果能先预测蛋白质乳酸化位点，后面的研究将事半功倍。本文将讲解如何预测蛋白质乳酸化位点。

Lactylation is a newly discovered protein modification related to metabolism, which refers to the enzymatic transfer of lactyl groups derived from lactate to protein lysine residues1. Histone lactylation plays an important role in regulating gene transcriptional activity. Non histone lactylation is involved in regulating protein activity and interactions. The lactylation writer protein (p300) promotes protein lactylation, while the lactylation eraser protein (HDAC1-3/SIRT1-3) inhibits protein lactylation. If protein lactylation sites could be predicted, subsequent research would be twice as efficient. This article shows how to predict lactylation sites on proteins.

利用DeepKla预测蛋白质乳酸化位点步骤如下：

The steps for predicting lactylation sites on proteins by DeepKla are as follows:

1 从http://lin-group.cn/server/DeepKla/index.html网址进入DeepKla

1 Enter DeepKla from http://lin-group.cn/server/DeepKla/index.html

2点击“Web-Server”

2 Click “Web-Server”

3 以FASTA格式输入蛋白质（人DICER1）序列，提交

3 Enter protein sequence (Human DICER1) in FASTA format, submit

4 查看结果

4 View results

5选择位点（PTM score≥75%）

5 Choose sites (PTM score≥75%)