如何预测tsRNA的靶基因如何预测tsRNA的靶基因 How to predict targets of tsRNA 转运RNA(tRNA)衍生的tsRNA(tRNA-derived small RNA)是一类新型的小非编码RNA(small non-coding RNA, sncRNA),参与调控mRNA干扰、稳定性和翻译。因此,靶基因预测对于tsRNA的研究十分重要。本文将讲解如何预测tsRNA的靶基因。 Transfer RNA (tRNA)-derived small RNA (tsRNA) is a novel type of small non-coding RNA (sncRNA), contributing to mRNA interference, stability and translation. Therefore, target prediction is critical for studying tsRNA. 对于已知的tsRNAs,靶基因预测步骤如下: For known tsRNAs, the steps of target prediction are as follows: 1从https://rna.sysu.edu.cn/tsRFun/index.php网址进入tsRFun 1 Enter tsRFun from https://rna.sysu.edu.cn/tsRFun/index.php
2点击tsRNetwork 2 Click tsRNetwork
3输入tsRNA id/名称,搜索 3 Enter tsRNA id/name, search
4查看结果 4 View results
对于新发现的tsRNAs,靶基因预测步骤如下: For novel tsRNAs, the steps of target prediction are as follows: 1从https://mirdb.org/index.html网址进入miRDB 1 Enter miRDB from https://mirdb.org/index.html
2点击Custom Prediction 2 Click Custom Prediction
3选择物种(如人类)以及提交核酸片段类型(miRNA Sequence) 3 Select Species (Human) and Submission type (miRNA Sequence)
4输入tsRNA序列,搜索 4 Enter tsRNA sequence, Go
5检索预测结果 5 Retrieve prediction result
6查看结果 6 View results
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