如何预测miRNA的靶基因

如何预测miRNA的靶基因

How to predict target of miRNA

        微小RNA（miRNA）是一类小非编码RNA（sncRNA），参与调控mRNA干扰和降解。因此，靶基因预测对于miRNA的研究十分重要。本文将讲解如何预测miRNA的靶基因。

Micro RNA (miRNA) is a type of small non-coding RNA (sncRNA), contributing to mRNA interference and degradation. Therefore, target prediction is critical for studying miRNA.

对于已知的miRNAs，靶基因预测步骤如下：

For known miRNAs, the steps of target prediction are as follows:

1从https://www.targetscan.org/vert_80/网址进入TargetScan

1 Enter TargetScan from https://www.targetscan.org/vert_80/

2选择物种

2 Select a species

3输入miRNA名称，提交

3 Enter a miRNA name, submit

4查看结果

4 View results

5或输入基因名称，提交

5 Enter a gene name, submit

6查看结果

6 View results

对于已知的miRNAs，还可以通过ENCORI/Starbase预测靶基因：

For known miRNAs, targets could be predicted by ENCORI/Starbase as well:

1从https://rnasysu.com/encori/index.php网址进入ENCORI/Starbase

1 Enter ENCORI/Starbase from https://rnasysu.com/encori/index.php

2 点击miRNA-target，选择miRNA-mRNA

2 Click miRNA-target，select miRNA-mRNA

3选择物种

3 Select a species

4输入miRNA名称，快速搜索

4 Enter a miRNA name, quick search

5查看结果

5 View results

6或输入靶基因名称，快速搜索

6 Enter a target name, quick search

7查看结果

7 View results

对于新发现的miRNAs，靶基因预测步骤如下：

For novel miRNAs, the steps of target prediction are as follows:

1从https://mirdb.org/index.html网址进入miRDB

1 Enter miRDB from https://mirdb.org/index.html

2点击Custom Prediction

2 Click Custom Prediction

3选择物种（如人类）以及提交核酸序列类型（miRNA Sequence）

3 Select Species (Human) and Submission type (miRNA Sequence)

4输入miRNA序列，搜索

4 Enter miRNA sequence, Go

5检索预测结果

5 Retrieve prediction result

6查看结果

6 View results