如何预测蛋白质与DNA的互作

如何预测蛋白质与DNA的互作

How to predict the interaction of protein and DNA

转录因子与靶基因启动子结合是最常研究的蛋白质与DNA的互作。本文通过探索TP53基因启动子上人STAT3结合位点讲解如何预测蛋白质与DNA的互作。

The association between transcription factor and targeted gene promoter is the most commonly studied protein-DNA interaction. This article shows how to predict protein-DNA interaction by exploring the binding sites of human STAT3 on TP53 gene promoter.

通过JASPAR预测蛋白质与DNA的互作：

The protein-DNA interaction could be predicted by JASPAR:

1 从https://jaspar.elixir.no/网址进入JASPAR

1 Enter JASPAR from https://jaspar.elixir.no/

2 选择类群(脊椎动物亚门)，点击

2 Select a taxonomic group (Vertebrata), click

3 选择物种（人类），输入STAT3，搜索

3 Select a species (Homo sapiens), input STAT3，search

4选择STAT3 profile(s)，在“Scan”输入TP53基因启动子序列，设置分数阈值，扫描（Scan）

4 Select STAT3 profile(s), input the sequence of TP53 gene promoter in “Scan”, set up score threshold, Sacn

5 获取结果

5 Obtain results