如何预测RNA与DNA的互作

如何预测RNA与DNA的互作

How to predict the interaction of RNA and DNA

        长链非编码RNA（lncRNA）可通过结合靶基因启动子调控靶基因转录。因此，lncRNA与靶基因启动子互作的预测对于lncRNA功能研究十分重要。本文将讲解如何预测lncRNA与靶基因启动子的互作。

Long non-coding RNA (lncRNA) could modulate targeted gene transcription by binding with its promoter. Therefore, prediction of the interaction of lncRNA and targeted gene promoter is crucial for studying lncRNA functions. This article shows how to predict the interaction of lncRNA and targeted gene promoter.

通过LongTarget预测lncRNA与靶基因启动子的互作：

The interaction of lncRNA and targeted gene promoter could be predicted by LongTarget:

1从http://www.gaemons.net/LongTarget网址进入LongTarget

1 Enter LongTarget from http://www.gaemons.net/LongTarget

2 选择物种，然后通过定义一个基因组区域输入DNA序列，检索

2 Select a species，and then input DNA Sequence by defining a genomic region，retrieve

3 或者粘贴一个DNA序列

3 Or paste a DNA sequence

4或者选择一种物种，用于在全基因组范围内预测输入的lncRNA在所有基因5000 bp启动子区域内的结合位点。

4 Or choose a species for genome-wide predicting the inputted lncRNA’s DNA binding sites in 5000 bp promoter regions of all transcripts.

5输入lncRNA序列

5 Input lncRNA sequence

6选择碱基配对规则集

6 Select a base pairing rule set

7设定参数

7 Set up parameters

8 选择一个程序，提交，获取工作ID

8 Select a program，submit，obtain job ID

9 通过工作ID查询结果

9 Retrieve result by job ID

10 下载结果

10 Download results