类泛素化修饰（UFMylation）

类泛素化修饰（UFMylation）

类泛素化是一种新近发现的蛋白质修饰¹。与泛素化类似，类泛素因子UFM1以前体形式被合成，经过UfSP1或UfSP2剪切，形成活化的UFM1-G83后被一个特定的E1样活化酶（UBA5）激活，转移到E2样结合酶（UFC1）上，随后UFC1和E3样连接酶（UFL1，又称NLBP或RCAD）共同识别靶蛋白并协助UFM1与靶蛋白结合，最后UFM1-靶蛋白共价结合体可以被UfSP1或UfSP2剪切，释放UFM1参与新的循环（图1）²。类泛素化通过调控蛋白质稳定性和互作来影响内质网应激、DNA损伤反应和红系发育等生理过程。

UFMylation is a newly discovered protein modification. Similar to ubiquitination, the precursor form of ubiquitin-like factor UFM1 is synthesized and cleaved by UfSP1 or UfSP2 to form activated UFM1-G83, which is then activated by a specific E1-like activating enzyme (UBA5) and transferred to E2-like conjugating enzyme (UFC1). Subsequently, UFC1 and E3-like ligase (UFL1, also known as NLBP or RCAD) jointly recognize the target protein and facilitate the specific binding between UFM1 and target protein. Finally, the UFM1-target protein covalent ligand can be cleaved by UfSP1 or UfSP2, releasing UFM1 to participate in a new cycle (Fig. 1) ². UFMylation contributes to endoplasmic reticulum (ER) stress, DNA damage response and erythroid development by regulating protein stability and interaction.

图1 类泛素化机制示意图²

Fig.1 Schematic diagram of molecular mechanisms for UFMylation²

                                                               检测方法（Methods）

客户提供的材料与信息（Materials and information provided by customers）：

1、目的蛋白抗体或信息（Target protein antibodies or information）；

2、细胞样本（Cells）：不低于2×10^7个细胞（No less than 2×10^7 cells）；

3、组织样本（Tissues）：不低于1g（No less than 1g）。

参考文献（References）

1    Liu, J. et al. UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination. Nat Cell Biol 22, 1056-1063 (2020). https://doi.org:10.1038/s41556-020-0559-z

2    Jing, Y., Mao, Z. & Chen, F. UFMylation System: An Emerging Player in Tumorigenesis. Cancers (Basel) 14 (2022). https://doi.org:10.3390/cancers14143501