拟素化修饰（Neddylation）

拟素化修饰（Neddylation）

拟素化是新近发现的一种类似泛素化的蛋白质修饰，指在一系列特殊的酶作用下泛素样分子NEDD8与靶蛋白赖氨酸残基共价结合的过程¹。拟素化是一个动态可逆的过程，具体如下：首先NEDP1对 NEDD8前体进行水解以暴露C端甘氨酸，随后E1激活酶（NEA1/APPBP1-UBA3复合物）以ATP依赖的方式结合并激活NEDD8，接着激活的NEDD8从E1传递到E2结合酶（UBC12 /UBE2F）上，然后E2和E3连接酶（RBX1/RBX2/MDM2）共同识别靶蛋白后协助NEDD8和靶蛋白的特异性结合，最后去拟素化酶将靶蛋白赖氨酸残基与NEDD8解离，解离的NEDD8又可以到下一个拟素化过程中去（图1）²。拟素化通过调控蛋白质稳定性、构象、活性和亚细胞定位来众多生理过程。

Neddylation is a newly discovered protein modification similar to ubiquitination, which refers to the process of covalent binding between ubiquitin-like molecule NEDD8 and target protein lysine residues under a series of special enzyme interactions. Neddylation is a dynamic and reversible process, as follows: first, NEDP1 hydrolyzes the precursor of NEDD8 to expose C-terminal glycine. Then E1 activating enzyme (NEA1/APPBP1-UBA3 complex) binds and activates NEDD8 in an ATP dependent manner. Next, the activated NEDD8 is transferred from E1 to E2 conjugating enzyme (UBC12/UBE2F). Subsequently, E2 and E3 ligases (RBX1/RBX2/MDM2) jointly recognize the target protein and facilitate the specific binding of NEDD8 to the target protein. Finally, the deneddylase dissociates the target protein lysine residue from NEDD8, and the separated NEDD8 would go on to the next neddylation process (Fig.1)². Neddylation contributes to numerous physiological processes by regulating protein stability, conformation, activity, and subcellular localization.

图1 拟素化机制示意图²

Fig.1 Schematic diagram of molecular mechanisms for neddylation²

                                                              检测方法（Methods）

客户提供的材料与信息（Materials and information provided by customers）：

1、目的蛋白抗体或信息（Target protein antibodies or information）；

2、细胞样本（Cells）：不低于2×10^7个细胞（No less than 2×10^7 cells）；

3、组织样本（Tissues）：不低于1g（No less than 1g）。

参考文献（References）

1    Enchev, R. I., Schulman, B. A. & Peter, M. Protein neddylation: beyond cullin-RING ligases. Nat Rev Mol Cell Biol 16, 30-44 (2015). https://doi.org:10.1038/nrm3919

2    Mao, H., Lin, X. & Sun, Y. Neddylation Regulation of Immune Responses. Research (Wash D C) 6, 0283 (2023). https://doi.org:10.34133/research.0283