如何预测非编码RNA的靶miRNA

如何预测非编码RNA的靶miRNA

How to predict targeted miRNA of ncRNAs

        多种非编码RNA(ncRNA)，包括长链非编码RNA（lncRNA）、环状RNA（circRNA）和小非编码RNA（sncRNA），都通过与微小RNA（miRNA）互作发挥作用。因此，靶miRNA预测对于非编码RNA的研究十分重要。本文将讲解如何预测非编码RNA的靶miRNA。

Numerous non-coding RNAs (ncRNAs) including long non-coding RNA (lncRNA), circular RNA (circRNA) and small non-coding RNA (sncRNA) exert their functions by interacting with miRNA. Therefore, prediction of targeted miRNA is critical for studying ncRNAs. This article shows how to predict targeted miRNA of ncRNAs.

对于已知的lncRNA/circRNA/sncRNA，靶miRNA预测步骤如下：

For known lncRNA/circRNA/sncRNA, the steps of targeted miRNA prediction are as follows:

1从https://rnasysu.com/encori/index.php网址进入ENCORI/Starbase

1 Enter ENCORI/Starbase from https://rnasysu.com/encori/index.php

2 点击“miRNA-target”，选择“miRNA-lncRNA/circRNA/sncRNA”

2 Click “miRNA-target”，select “miRNA-lncRNA/circRNA/sncRNA”

3选择物种

3 Select a species

4输入lncRNA/circRNA/sncRNA名称，快速搜索

4 Enter the lncRNA/circRNA/sncRNA name, quick search

5查看结果

5 View results

6或输入靶miRNA名称，快速搜索

6 Or enter the name of targeted miRNA, quick search

7查看结果

7 View results

对于已知的circRNA，还可以通过Circular RNA Interactome预测靶miRNA：

For known circRNA, targeted miRNA could be predicted by Circular RNA Interactome as well:

1从https://circinteractome.nia.nih.gov/index.html网址进入Circular RNA Interactome

1 Enter Circular RNA Interactome from https://circinteractome.nia.nih.gov/index.html

2 点击“miRNA Target Sites”

2 Click “miRNA Target Sites”

3输入circRNA名称，点击“miRNA Target Search”

3 Enter the circRNA name, click “miRNA Target Search”

4查看结果

4 View results

对于新发现的lncRNA/circRNA/sncRNA，靶miRNA预测步骤如下：

For novel lncRNA/circRNA/sncRNA, the steps of targeted miRNA prediction are as follows:

1从https://mirdb.org/index.html网址进入miRDB

1 Enter miRDB from https://mirdb.org/index.html

2点击Custom Prediction

2 Click Custom Prediction

3选择物种（如人类）以及提交核酸序列类型（mRNA Target Sequence）

3 Select Species (Human) and Submission type (mRNA Target Sequence)

4输入lncRNA/circRNA/sncRNA序列，搜索

4 Enter the lncRNA/circRNA/sncRNA sequence, Go

5检索预测结果

5 Retrieve prediction result

6查看结果

6 View results