甲基化修饰（Methylation）

        RNA甲基化是指将甲基基团共价添加到RNA核苷酸上的修饰方式，对RNA稳定性、定位、运输、剪切和翻译等发挥重要的作用。根据修饰位置，RNA甲基化类型分为m6A，m1A，m3C，m5C，m7G和2’-O-me等。RNA甲基化是一个动态调控过程，三类关键蛋白参与其中：甲基转移酶（Writer），负责将甲基基团共价添加到RNA核苷酸上；去甲基化酶‌（Eraser），负责把甲基基团从RNA核苷酸上移除；甲基识别蛋白（Reader），负责识别甲基基团并发挥作用¹。不同RNA甲基化类型的调控蛋白可见表1。

RNA methylation refers to the modification process where methyl groups are covalently added to RNA nucleotides, playing a crucial role in RNA stability, localization, transportation, splicing, and translation. Based on the modification location, RNA methylation types are classified into m6A, m1A, m3C, m5C, m7G, and 2'-O-me. RNA methylation is a dynamically regulated process involving three key proteins: Writer, responsible for covalently adding methyl groups to RNA nucleotides; Eraser, responsible for removing methyl groups from RNA nucleotides; and Reader, responsible for recognizing and exerting the function of methyl groups¹. The regulatory proteins for different RNA methylation types are shown in Table 1.

图1 RNA甲基化机制示意图¹

Fig.1 Schematic diagram of molecular mechanisms for RNA methylation¹

                                                                 表1 RNA甲基化调控蛋白

                                                                 Table 1 The regulator proteins of RNA methylations

                                                              检测方法（Methods）

应用（Application）

1. 确定靶RNA甲基化（Identification of target RNA methylation）；

2. RNA修饰测序（Sequencing of modified RNAs）。

客户提供的材料与信息（Materials and information provided by customers）：

1、目的RNA信息（Target RNA information）；

2、细胞样本（Cells）：不低于2×10^7个细胞（No less than 2×10^7 cells）；

3、组织样本（Tissues）：不低于1g（No less than 1g）。

参考文献（Reference）

1    Li, Y. et al. The role of RNA methylation in tumor immunity and its potential in immunotherapy. Mol Cancer 23, 130 (2024). https://doi.org/10.1186/s12943-024-02041-8