如何预测蛋白质巴豆酰化位点

如何预测蛋白质巴豆酰化位点

How to predict crotonylation sites on proteins

       巴豆酰化是一种动态可逆的蛋白质修饰，指将巴豆酰基团从巴豆酰辅酶 A 转移到蛋白质特定赖氨酸残基上的过程。巴豆酰化参与基因转录调控和DNA损伤修复，与肿瘤、胚胎发育、心血管疾病等密切相关。写入酶（p300/CBP/GCN5/HAT1/TIP60/KAT7/PCAF/MOF）催化蛋白质巴豆酰化，擦除酶（SIRT1-3/SIRT6-7/FOSIR5/HDAC1-3/HDAC6-8）则去除蛋白质巴豆酰化，而识别蛋白（TAF14/YEATS2/AF9/MOZ/DPF2/TAF1）则识别巴豆酰化蛋白质。在研究蛋白质巴豆酰化之前，如果能先预测蛋白质巴豆酰化位点，后面的研究将事半功倍。本文将讲解如何预测蛋白质巴豆酰化位点。

Crotonylation is a dynamically reversible protein modification, referring to the process of transferring crotonyl groups from crotonyl-CoA to specific lysine residues on proteins. Crotonylation is involved in gene transcription regulation and DNA damage repair, and is closely related to tumors, embryonic development, cardiovascular diseases and so on. The writers (p300/CBP/GCN5/HAT1/TIP60/KAT7/PCAF/MOF) catalyze protein crotonylation, while the erasers (SIRT1-3/SIRT6-7/FOSIR5/HDAC1-3/HDAC6-8) remove protein crotonylation, and readers (TAF14/YEATS2/AF9/MOZ/DPF2/TAF1) recognize crotonylated proteins. If protein crotonylation sites could be predicted, subsequent research would be twice as efficient. This article shows how to predict crotonylation sites on proteins.

利用Deep-Kcr预测蛋白质巴豆酰化位点，步骤如下：

The steps for predicting crotonylation sites on proteins by Deep-Kcr are as follows:

1 从http://lin-group.cn/server/Deep-Kcr/网址进入Deep-Kcr

1 Enter Deep-Kcr from http://lin-group.cn/server/Deep-Kcr/

2点击“Web-Server”

2 Click “Web-Server”

3 以FASTA格式输入蛋白质（人SUCLG2）序列，提交

3 Enter protein sequence (Human DICER1) in FASTA format, submit

4 查看结果

4 View results

另外，还可以利用CPLM（Compendium of Protein Lysine Modifications）查看已知的蛋白质巴豆酰化位点，步骤如下：

Additionally, CPLM also could be used to view known crotonylation sites on proteins, and steps are as follows:

1从https://cplm.biocuckoo.cn/index.php网址进入CPLM

1 Enter CPLM from https://cplm.biocuckoo.cn/index.php

2输入基因名称（SUCLG2），提交

2 Input gene name (SUCLG2), submit

3 选择物种（人），点击“CPLM ID”

3 Select species (Homo sapiens), click “CPLM ID” to view results

4选择修饰类型（Crotonylation），查看结果

4 Select modification type (Crotonylation), view results

5 点击“View”查看详情

5 Click “View” for details