如何预测蛋白质与蛋白质的互作

如何预测蛋白质与蛋白质的互作

How to predict the interaction of protein and protein

        蛋白质互作对于蛋白质发挥功能至关重要。因此，预测蛋白质与蛋白质的互作对于机制研究十分重要。本文将讲解如何预测蛋白质与蛋白质的互作。

The interaction of protein and protein is crucial for functional performance of proteins. Therefore, prediction of protein-protein interaction is important for mechanical study. This article shows how to predict the interaction of protein and protein.

对于已知的蛋白质与蛋白质的互作，可以利用NCBI-Gene挖掘，步骤如下：

For known protein-protein interaction, NCBI-Gene could be used to dig, and steps are as follows:

1 从https://www.ncbi.nlm.nih.gov/网址进入NCBI

1 Enter NCBI from https://www.ncbi.nlm.nih.gov/

2 点击“All Databases”，选择“Gene”

2 Click “All Databases”，select “Gene”

3 输入基因名称（TP53），搜索

3 Input the gene name (TP53)，search

4 选择物种（人类）

4 Select an organism (Homo sapiens)

5 查看结果，点击基因（TP53）

5 View results，click the gene (TP53)

6 找到“Interactions”，查看互作的蛋白质

6 Find “Interactions”，view interacted proteins

对于已知的蛋白质与蛋白质的互作，还可以利用BioGRID挖掘，步骤如下：

For known protein-protein interaction, BioGRID also could be used to dig, and steps are as follows:

1 从https://thebiogrid.org/网址进入BioGRID

1 Enter BioGRID from https://thebiogrid.org/

2 输入蛋白质名称（TP53），选择物种（人类），搜索

2 Input the gene name (TP53)，select an organism (Homo sapiens), search

3 查看结果

3 View results

对于未知的蛋白质与蛋白质的互作，可以利用PEPPI进行预测，步骤如下：

For unknown protein-protein interaction, PEPPI could be used to predict, and steps are as follows:

1 从https://aideepmed.com/PEPPI/网址进入PEPPI

1 Enter PEPPI from https://aideepmed.com/PEPPI/

2 输入蛋白质序列

2 Input protein sequences

3 输入科研单位邮箱，免费或商业邮箱无效

3 Input the institutional email，email from free or commercial email accounts is invalid

4 点击“Run PEPPI”，结果发送到邮箱

4 Click “Run PEPPI”，and results will be sent by email

5 查看结果

5 View results